1Plant Protection Research Department, Yazd Agricultural and Natural Resources Research and Education Centre, Yazd, Iran
2Plant Protection Research Department, Chaharmahal and Bakhtiari Agricultural and Natural Resources Research and Education Centre, Shahrekord, Iran
3Department of Agricultural and Food Sciences, Alma Mater Studiorum - University of Bologna, Italy
*Corresponding author e-mail: Seyyed Alireza Esmaeilzadeh-Hosseini (phytoplasma.iran@gmail.com)
Online published on 23 May, 2023.
A Cressa cretica witches’ broom disease was observed in Ardakan (Yazd province, Iran) during 2018–2021. Total DNAs extracted from eighteen symptomatic and four asymptomatic plants were subjected to nested PCR assays using primers amplifying the phytoplasma 16S ribosomal DNA. Expected length of PCR amplification products was detected in all symptomatic plant samples but not in those asymptomatic. RFLP analysis of the R16F2n/R16R2 amplicons showed mixed infection of phytoplasmas enclosed in 16SrXXIX and 16SrI groups.
Phytoplasma diseases, 16SrI, 16SrXXIX, Molecular detection, RFLP analyses
