Phytopathogenic Mollicutes
SCOPUS
  • Year: 2023
  • Volume: 13
  • Issue: 1

Detection and identification of ‘Candidatus Phytoplasma rubi’ and viruses in Rubus idaeus using total RNA

  • Author:
  • Jana Franova1,*, Jaroslava Pribylova1, Rostislav Zemek2, Jiunn Luh Tan2,3, Zhibo Hamborg4, Dag-Ragnar Blystad4, Ondrej Lenz1, Igor Koloniuk1
  • Total Page Count: 2
  • Page Number: 89 to 90

1Biology Centre CAS, Institute of Plant Molecular Biology, Ceske Budejovice, Czech Republic

2Biology Centre CAS, Institute of Entomology, Ceske Budejovice, Czech Republic

3Faculty of Science, University of South Bohemia, Ceske Budejovice, Czech Republic

4Division of Biotechnology and Plant Health, Norwegian Institute of Bioeconomy Research, As, Norway

*Corresponding author e-mail: Jana Franova (jana@umbr.cas.cz)

Online published on 23 May, 2023.

Abstract

In a survey for viruses in a production field of raspberry in central Bohemia, several plants had symptoms reminiscent of phytoplasma and virus. The samples were analysed using PCR and Sanger sequencing. ‘Candidatus Phytoplasma rubi’ was identified in seven raspberry bushes (five plants with symptoms resembling phytoplasma infection, one plant with diffuse mosaic and one asymptomatic plant). A total of 31 plants tested positive for viruses (mainly raspberry leaf mottle virus and raspberry bushy dwarf virus).

Keywords

Phytoplasma, PCR, Sanger sequencing, Virus, Raspberry