1Department of Molecular and Cellular Engineering, Jacob School of Biotechnology and Bioengineering, Sam Higginbottom University of Agriculture, Technology and Sciences, Prayagraj - 211007, Uttar Pradesh, India
2Institute of Agriculture and Natural Sciences, DDU Gorakhpur University, Gorakhpur - 273009, Uttar Pradesh, India
3Department of Plant Pathology, Acharya Narendra Deva University of Agriculture & Technology, Kumarganj, Ayodhya, 224229, Uttar Pradesh, India
*Corresponding author e-mail: Surabhi Mitra (surabhimitra19@gmail.com)
Online published on 23 May, 2023.
Little leaf and witches’ broom symptoms were observed on Tecoma stans plants at the AND University campus, Ayodhya, India, during 2021–2022. A 1,250 bp product was amplified from DNA extracted from symptomatic T. stans plant samples subjected to nested PCR assays with P1/P7 and R16F2n/R16R2 primers. Sequence analysis of the 16Sr RNA gene product through phylogeny and virtual RFLP analyses of the T. stans phytoplasma strain allowed its identification as ‘Candidatus Phytoplasma australasia’ (16SrII-D). This is a first record of phytoplasma presence in T. stans.
Trumpet flower, Yellow bells, 16S rRNA gene, 16SrII-D phytoplasma subgroup
