Utilization of dnaB gene for characterisation of phytoplasmas associated with toria, brinjal and Phlox in India

In the present study a new set of primers was designed, which amplified 961 bp fragment of dnaB gene. The dnaB gene-based primers were successfully utilized for identification of phytoplasmas belonging to 16SrVI and 16SrXI groups associated with phlox little leaf and yellowing, toria phyllody and brinjal little leaf. This dnaB gene primer set could be used as an alternative PCR-based diagnostic tool for rapid and specific assay for both specific detecting and confirming the presence of phytoplasmas that affiliate with 16SrVI and 16SrXI ribosomal groups.