1University of Chile, Facultad de Ciencias Agronómicas, Departamento de Sanidad Vegetal, Santiago, Chile
2IANSA, Departamento de Investigación y Desarrollo, Chillán, Chile
*Corresponding author e-mail: Nicola Fiore (nfiore@uchile.cl)
Online published on 2 January, 2015.
Due to the sporadic occurrence of yellow wilt of sugar beet disease throughout the last three years, a survey was carried out in two regions where the Chilean sugar beet production is concentrated. The tuf and 16S rRNA genes were used for phytoplasma detection and identification. Virtual restriction fragment length polymorphism analyses, cloning, and sequencing identified the presence of a phytoplasma belonging to the ribosomal subgroup 16SrIII-J.
Yellow wilt of sugar beet, nested-PCR, tuf gene, 16S rRNA gene, RFLP, sequencing
