1College of Biotechnology, Sardar Vallabhbhai Patel University of Agriculture and Technology, Meerut, India
2Plant Virology Unit, Division of Plant Pathology, Indian Agricultural Research Institute, New Delhi, India
*Corresponding author e-mail: Jitender Singh (jeets-80@rediffmail.com)
Online published on 2 January, 2015.
Symptoms associated with yellows disease of eggplants were observed in fields of Meerut district of India. Total DNA extracted from symptomatic and asymptomatic plants was subjected to nested-PCR assay using phytoplasma 16S rDNA primers and generic PCR with universal begomovirus coat-protein (CP) gene primers. Amplicons of expected size were obtained from symptomatic plants in both PCR assays. No amplifications were observed from asymptomatic plants. The eggplant phytoplasma was identified as a member of group 16SrVI, while the begomovirus showed 99% of CP gene identity with other whitefly transmitted begomoviruses.
Eggplant, brinjal, phytoplasmas, begomoviruses, nested-PCR
