Phytopathogenic Mollicutes
SCOPUS
  • Year: 2017
  • Volume: 7
  • Issue: 2

16SrIII-J phytoplasmas infecting lettuce and Swiss chard crops in Chile

  • Author:
  • Nicolás Quiroga1,2,4, Marcelo Bustamante3,4, Camila Gamboa3,4, Javiera Molina3,4, Alan Zamorano1,4, Nicola Fiore1,4,
  • Total Page Count: 4
  • Page Number: 91 to 94

1Universidad de Chile, Facultad de Ciencias Agronómicas, Departamento de Sanidad Vegetal

2Universidad de Chile, Campus Sur, Ph.D. Program in Agricultural and Veterinary Sciences

3Universidad de Chile, Facultad de Ciencias Agronómicas, Master Program in Agricultural Sciences

4Avenida Santa Rosa, 11315, La Pintana, Santiago, Chile

*Corresponding author e-mail: Nicola Fiore, nfiore@uchile.cl

Online published on 24 January, 2018.

Abstract

During spring 2016 commercial lettuce and Swiss chard plants, exhibiting disease symptoms suggestive of possible phytoplasma infection, were collected together with asymptomatic plants in Metropolitana and Arica y Parinacota regions of Chile, respectively. Nucleic acids extracted from leaf midribs were used for phytoplasmas detection. Nested polymerase chain reaction allowed the amplification of bands of the expected size only from symptomatic samples. Restriction fragment length polymorphism analysis, cloning, and sequencing allowed the identification of 16SrIII-J phytoplasmas in both plant species.

Keywords

horticultural species, nested-PCR, cloning, RFLP, sequencing