Phytopathogenic Mollicutes
SCOPUS
  • Year: 2019
  • Volume: 9
  • Issue: 1

New diseases associated with 16SrIII-J phytoplasmas in Chile

  • Author:
  • Nicolás Quiroga1,2,, Gabriela Medina1, Alan Zamorano1, Ivette Acuña3, Rosa Piña4, Nicola Fiore1
  • Total Page Count: 2
  • Page Number: 15 to 16

1Universidad de Chile, Facultad de Ciencias Agronómicas, Departamento de Sanidad Vegetal, La Pintana, Santiago, Chile

2Universidad de Chile, Campus Sur, PhD Program in Agricultural and Veterinary Sciences, La Pintana, Santiago, Chile

3Instituto de Investigaciones Agropecuarias, INIA-Remehue, Osorno, Chile

4Pinto Piga Seeds SA, El Monte, Chile

*Corresponding author e-mail: Nicola Fiore (nfiore@uchile.cl)

Online published on 25 July, 2019.

Abstract

Several plants of potato, cactus pear and carrot showing symptoms of presumable phytoplasma infection, were collected in Metropolitana and Lagos regions of Chile. Nucleic acid was extracted from the leaf midribs and used for phytoplasma detection. The amplification products of the expected size with nested polymerase chain reaction on tuf and 16S rRNA genes were obtained only from symptomatic samples. Cloning, sequencing and virtual restriction fragment length polymorphism analysis, allowed the identification of the 16SrIII-J phytoplasma presence in all plant species tested.

Keywords

Potato, cactus pear, carrots, nested-PCR, cloning, RFLP, sequencing