1Alma Mater Studiorum, University of Bologna, Department of Agricultural and Food Sciences, Bologna, Italy
2Institute for Sustainable Plant Protection-National Research Council of Italy, IPSP-CNR, Turin, Italy
*Corresponding author e-mail: Claudio Ratti (claudio.ratti@unibo.it)
Online published on 25 July, 2019.
When high numbers of analyses are required a rapid sample preparation method coupled to RT-qPCR allows to reduce the time and the costs for the phytoplasma detection in plant samples. A comparison between DNA and RNA contribution to qPCR detection revealed the higher input of the latter ensuring the maintenance of the sensitivity and specificity of the assay. The protocol has been validated for the detection and quantification of ‘Candidatus Phytoplasma mali’, ‘Ca. P. pyri’, ‘Ca. P. prunorum’, ‘Ca. P. solani’ and “flavescence dorée” phytoplasma by qPCR, RT-qPCR, ddPCR and ddRT-PCR techniques based on TaqMan chemistry.
DNA and RNA extraction, crude extract, phytoplasmas, detection
