1Alma Mater Studiorum, University of Bologna, Department of Agricultural and Food Sciences, Bologna, Italy
2Diagnostic group, Agricultural Biotechnology Research Institute, NARIC, Godollo, Hungary
*Corresponding author e-mail: Eva Varallyay (varallyay.eva@abc.naik.hu)
Online published on 25 July, 2019.
Aster yellows phytoplasma strain Hyd35 (16SrI-B) in micropropagated periwinkle shoots in collection was used to produce infected plants in pots that were separated according to the diverse symptomatology i.e. phyllody and witches’ broom. Small RNA highthroughput sequencing (HTS) was then used to determine the small RNA pattern of these plants. Bioinformatics analysis revealed the presence of expression changes of different miRNA classes and the presence of phytoplasma derived small RNAs. These results could complement previous studies and serve as a starting point for small RNA omics in phytoplasma research.
Aster yellows, Catharanthus roseus, miRNA, small RNA, HTS
