Department of Plant Pathology, Assam Agricultural University, Jorhat, Assam, India
*Corresponding author e-mail: Subrata Bora (subratabora107@gmail.com)
Online published on 25 July, 2019.
The possible association of phytoplasmas with the Banana bunchy top virus (BBTV) and little leaf symptoms in banana was investigated. Symptomatology showed presence of characteristic little leaf symptoms in BBTV infected banana plants. Six banana samples showing little leaf were assayed for the presence of phytoplasmas and their association with BBTV using polymerase chain reaction (PCR). Five out of thr six tested genotypes were found positive to phytoplasmas while all were positive to BBTV. The 1.5 kb product from the amplification of the 16S rRNA in PCR with primer pair P1/P6 and about 1.25 kb product in nested PCR assay confirmed the association of phytoplasmas with BBTV infected banana genotypes.
Banana bunchy top disease, mixed infection, PCR, plant disease
