1Universidad de Chile, Facultad de Ciencias Agronómicas, Departamento de Sanidad Vegetal, La Pintana, Santiago, Chile
2Universidad de Chile, Campus Sur, Ph. D. Program in Agricultural and Veterinary Sciences, La Pintana, Santiago, Chile
3University of Turin, DISAFA, Dipartimento di Scienze Agrarie, Forestali e Alimentari, Grugliasco, Torino, Italy
*Corresponding author e-mail: Nicola Fiore (nfiore@uchile.cl)
Online published on 25 July, 2019.
Three 16SrIII-J phytoplasma-positive vineyards were visited each 15 days from September 2017 to August 2018 to capture insects using entomological sweep nets and yellow sticky traps. Additionally weeds showing typical symptoms associated with the presence of phytoplasmas were also collected. The phytoplasma 16SrIII-J was detected for the first time in the leafhoppers Amplicephalus ornatus, A. pallidus and Bergallia sp., and in the weed Brassica campestris. Another phytoplasma classified as 16SrXI, was detected in one leafhopper belonging to the genus Paratanus. The identification of the 16SrXI ribosomal subgroup and the species of Bergallia sp. and Paratanus sp. are in progress, as well as the transmission trials with these two insects plus A. pallidus and A. ornatus.
Insects vector, GY, nested-PCR, RFLP, sequencing, weeds
