1Alma Mater Studiorum, University of Bologna, Department of Agricultural and Food Sciences, Bologna, Italy
2Agritest srl, Valenzano, Bari, Italy
*Corresponding author e-mail: Nicoletta Contaldo (nicoletta.contaldo2@unibo.it)
Online published on 25 July, 2019.
The development of methods for culturing phytoplasmas in artificial media suggested the evaluation of strategies to produce antibodies against a phytoplasma target. To this purpose, an aster yellows phytoplasma isolate obtained from field collected infected grapevine was used for the production of a polyclonal antiserum. It was then tested by ELISA and IFAS methods in order to check and evaluate its suitability for phytoplasma detection in plant samples.
Aster yellows isolate, polyclonal antiserum, DAS ELISA, IFAS
