Retrieval of Oocytes from Ovaries of Buffalo Cows Slaughtered in Abattoir

A total of 890 ovaries were collected from January to August 2006 and used for the present investigation. The mean number of visible follicles on the surface of left and right ovary was 3.87±0.23 and 3.66±0.28. The total number of oocyte yield in aspiration, dissection and slicing techniques were 3.20±0.16, 3.10±0.10 and 5.70±0.13, respectively. The total number of oocyte yield in follicular and luteal phases of estrous cycle was 5.31±0.12 and 3.88±0.13, respectively.

Among the oocytes recovered, 52.94, 25.88 and 21.18 per cent were good, fair and poor quality of oocytes, respectively. The mean diameters of good, fair and poor quality of oocytes were 135.65±2.52, 111.7±2.84 and 94.55±2.22 μ, respectively. The number of follicles present on the ovary was not significantly differed among the phases of estrous cycle. The mean number of oocytes retrieved was higher in slicing technique and from the ovaries under follicular phase of estrous cycle.