1Department of Veterinary Gynecology and Obstetrics, College of Veterinary and Animal, Sciences, Rajasthan University of Veterinary and Animal Sciences, Bikaner, Rajasthan, India, 334001
2Government Veterinary Hospital, Dhab Dhani, Haryana, India
3Department of Veterinary Anatomy and Histology, CVAS, Bikaner, Rajasthan, India
4Veterinary Officer Veterinary Polyclinic, Churu, Rajasthan, India
5Agriculture Finance Officer, Central Bank of India, Alot, Indore, Madhya Pradesh, India
*Corresponding author: gnpobs@gmail.com
Online published on 8 October, 2015.
In order to evaluate the effect of media on in vitro maturation, culturable grade buffalo oocytes were matured in vitro in three different media (Ham's F-10, Waymouth MB and TCM-199) with same supplements (5μg/ml FSH, 5μg/ml LH, 1μg/ml estradiol, 25 mM Hepes, 0.25 mM pyruvate and antibiotics). The overall mean culturable grade oocyte recovery was 3.12±0.20. At the end of experiment all oocytes in all groups were fixed and stained to evaluate the nuclear status. Oocytes were considered mature if they were at metaphase II. Significantly higher (P<0.01) proportion of oocytes were matured in vitro in Waymouth medium compared to Ham's F-10. In TCM-199, IVM rates were non-significantly higher compared to Ham's F-10 and non-significantly lower compared to Waymouth MB medium. It was concluded that Way mouth MB medium is the most appropriate medium for in, vitro maturation of buffalo oocytes followed by TCM-199 and Ham's F 10.
In vitro maturation, buffalo oocytes, follicles, in vitro embryo production
